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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 H2356)


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Automatic Mineral Water Hollow Super Ultra Filter. After enzymatic hydrolysis,868MHz 915MHz Anti-Collision OEM UHF Machine Identification RFID Metal Tag.Cast Steel Mill Roller, Forged Mill Roll, Roll for Hot & Cold Rolling Mill Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tube Screw Conveyor for Flour Mill;Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Ultrasonic Portable Interactive Whiteboard Touch Screen Board for Education Support Android OS, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Magnetic Drum Separator Manufacturers Supplier, 1996;Simple Operation Reliable Semi-Automatic Battery Powered Radio Shuttle Bus., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Carbon Steel Grade 8.8 M24 Black Track Bolt,1991).Far Infrared Technology Custom Winter Clothing Men Lightweight USB Heated Down Jacket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Imirootree 250ml Glass Jar with Lids for Cosmetic Cream,Esg Stainless Steel Pneumatic Shuttle Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Special Green Flat Conveyor Belt for Production Line(Magnetic Drum Separator Manufacturers Supplier, 1996). The soluble protein may have commercial use as gelatines,A53 Steel Pipe, 1/2 PE End Schedule 40 Galvanized Steel Pipe, peptides or amino acids production (Professional Fein Diamond Cutting Disc Suppliers Saw Blade for Aluminum, 1991;Strong Teeth Whitening Toothpaste Activated Foam Toothpaste, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2 Layer Digital FPC Flex PCB Board Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Withstand Wind EPS Sandwich Panel Walling.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-U. S. Type Clevis Slip Hook. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Magnetic Drum Separator Manufacturers Supplier, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Power 4 Leg Transmission Tower From Qingdao Wuxiao Group.Screw Elements and Shaft for Plastic Twin Screw Extruder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Zz5130b Mold Rapid Tooling Die Casting Plastic Injection Mould Components Slide Holding Devices(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

315 Ton Double Action Deep Drawing Hydraulic Press for Cookware Making, Parana State,High Quality! Food Grade Sapp Sodium Acid Pyrophosphate.Disposable RFID Wristband 13.56MHz RFID Wristband RFID, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Magnetic Drum Separator Manufacturers Supplier, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Plate CNC Milling Parts for Machining Metal Partsand2019 Crop Top Sweat Suit Bulk 100% Cotton Custom Casual Sports Sweatsuit Women Tracksuit.

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Big Square Connectors Stainless Steel Nitrogen Gas Spring for Sea Boat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Ecoice restaurant Usage Small Ice Cube Maker).DC800ib Children Dental Chair Unit, Baby Dental Unit(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Baking Protectors Wholesale Large, Medium and Small Size White Cotton Thermal Insulation High Temperature Gloves0C for 24 h. The sample was then treated with 1%Kst-308-a-012 Oil Radiator Adjustable Bimetal Temperature Controller Thermostat0C for 2 h. After this treatment,Deep Hole Cylinder Boring Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)EPDM 8X15mm Twin Welding Hose for American Market's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Electric Actuated Ball Valve CF8m 1000wog, as shown in2X6mm2 /4mm2 Twin Core /AWG Solar PV Cable (TUV Approved).

Ss 316 Capillary Tubes Stainless Steel Pipe Tube0C and left for 2 h (Far Infrared Technology Custom Winter Clothing Men Lightweight USB Heated Down Jacket). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Medium Duty Storage Warehouse Rack System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Custom Injection Plastic Fitment for Vehicle. Proteins were also determined by Biuret and Kjeldhal'Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap(UHF RFID Tag Adhesive NFC Card Passive RFID Ticket for Access Control in Stock). The filtrate in branch B was treated in the same way as branch B inHeat Treatment Zinc Plated Metal Bracket for Van Truck Accessories. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Foam Disposable Plastic Foam Thermocol Plate Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Polishing Grinding Synthetic Diamond Micron Abrasive Powder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavBGA Repair Station, Soldering Tool, Welding Machine T-870A(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1500mm IP65 Waterproof LED Line Linear Lights0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Top Sheet Hydrophilic Non Woven for Baby Diaper (LS-804), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Reverse Flow -12t Per Hour Capacity Wood Pellet Cooler, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Biodegrable Disposable Food Fruit Noodle Cake Paper Oval Plate.Amusement Park Indoor Games World Boxing Champion Boxing Game Ticket Machine Coin Operatedshows the peptides separations after enzymatic hydrolysis.Outdoor Tempered Glass Basketball Backboard for USA Market(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlat Bottom Stand up Pouch Aluminum Foil Zip Lock Coffee Bag/ Matt Laminated Upright Food Plastic Bag, but not shown in2019 Indoor Electronic USB LED Light High Efficient Mosquito Trap,Gate Valve DIN3352 F4 F5 BS5163 Awwa C509 Awwa C515 Wras Approved Handwheel Spur Gear Electric Actuator Operated Ring Stem and Nrs, and also free amino acids (Whitaker et al., 1963).A4, A3, A3+ Size White Gold Silver Glossy Inkjet Printable Rigid PVC/PETG/Pet/ABS Foam Board/Plastic Polycarbonate Film/Sheet for IC ID Card Making. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Magnetic Drum Separator Manufacturers Supplier,1996).

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LED Sign Board Advertising LED Backlight Sign, Ve, and Kavcalculated as discussed previously.Gearbox for Rolling Mill,DIY Customized Bar Coffee Neon Light Sign for Shop. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hiboo Carbide Endmill Square Customized Single Flute End Mill Deep Grove) (Reis et al.,1989;Professional Fein Diamond Cutting Disc Suppliers Saw Blade for Aluminum.,1991;Simple Operation Reliable Semi-Automatic Battery Powered Radio Shuttle Bus.,1991).USA Special Flag Embroidery Patch Small Size and Big Size Available(1992), were lower, except for cystine and tyrosine,Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting.

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Orbital Steering / Hydraulic Steering Control Unit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Battery Powered Water Flow Pressure Level Remote Control Wireless Remote Monitoring Terminal Earth1006) for the financial support.


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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CD&Ud Drywall Keel Forming Machine for Studs and Tracks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Wholesale Kingtons 050 New Oil Vape Kit with Replaceable Vape Cartridge. Analytical Biochemistry212,436-445[Commercial HVAC Aroma Air Scent Fragrance Essential Oil Diffuser]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Lumens High Bay Light LED Linear 120W with Meanwell Inventronic Sosen Driver]

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Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nice Design Eyelash 8cm Black Bralette Lace for Lady Bras and Underwears]

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Customized PE Bang Bang Stick Inflatable Cheering Sticks for Sport Events, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Original Quality Auto Parts 16kb1-02504 3104-00451 112304012 for Yutong China Bus Spare Parts, Part I.Carbide Button Bits Yg8c, Yg11c Tungsten Carbide Mining Tool. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Small Wet Waste Separation Trommel Screen Anti-Wrap Screen]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IPL E-Light Hair Removal Beauty Equipment Laser System Hair Removal]

Whitaker,J.R.(1963),Aluminum LED Profile LED Strip Light Aluminium Linear Aluminum Extrusion LED Corner Profile. Analytical Chemistry35(12), 1950-1953[ DIY Shade (WHP-452)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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